Programme

Course info Speaker bios

10 August — 15 August, 2018

1st Day

Introduction to CRISPR

09:00 — 09:15

Opening remarks (Prof. Dong-Yan Jin and Prof. Ben Berkhout)

09:15 — 10:30

Introduction to CRISPR (Dr. Bo Gao)

10:30 — 11:45

Cutting-edge CRISPR technologies (Dr. Alan Wong)

11:45 — 13:00

Bioinformatics of gRNA design (Prof. Qi Liu)

13:00 — 14:30

Lunch

14:30 — 17:00

Practical session 1: 1) Practical briefing. 2) gRNA design. 3) Transfection of CRISPR plasmids into HEK293 cells (Dr. Sam Yuen)

2nd Day

CRISPR in model systems

09:00 — 10:30

Genome editing in preimplantation embryos and in EPSCs (Prof. Pengtao Liu)

10:30 — 12:20

Organoids as a model system and their tools for genetic manipulation (Dr. Bon-Kyoung Koo)

12:20 — 14:30

Lunch

14:30 — 17:00

Practical Session 2: 1) Cloning of CRISPR construct. 2) Introduction to CRISPR library construction and other CRISPR resources (Dr. Sam Yuen)

3rd Day

Mechanism and delivery of CRISPR

09:00 — 10:30

Crystallography and HF-AFM of CRISPR-Cas9 (Prof. Osamu Nureki)

10:30 — 12:20

Workshop (Prof. Ben Berkhout and all invited speakers)

12:20 — 14:30

Lunch

14:30 — 17:00

Practical Session 3: 1) Harvest of genomic DNA. 2) PCR verification of CRISPR editing (Dr. Sam Yuen)

4th Day

Symposium on precision genome editing

08:30 — 08:45

Opening remarks (HKU representatives)

08:45 — 09:30

Bioinformatics of CRISPR editing (Dr. Qi Liu)

09:30 — 10:25

Molecular mechanism of CRISPR-Cas and structure-guided development of genome-editing tools towards medical applications (Prof. Osamu Nureki)

10:25 — 11:10

CRISPR editing in mice (Prof. Pengtao Liu)

11:10 — 11:55

CRISPR/Cas-assisted genetics in intestinal organoids (Dr. Bon-Kyoung Koo)

11:55 — 12:40

CRISPR-based genetic screens (Dr. Kosuke Yusa)

12:40 — 14:00

Lunch

14:00 — 14:45

Gene editing of the functional cancer genome (Dr. Sidi Chen)

14:45 — 15:40

The use of large scale functional genomic screens for the identification of novel drug targets and effective combination therapies (Dr. Roderick Beijersbergen)

15:40 — 16:25

CRISPR-Cas9 screening to identify cancer dependencies (Dr. Andrew Aguirre)

16:25 — 17:10

CRISPR-Cas genome editing technologies for therapeutics (Dr. Shengdar Tsai)

17:10 — 17:55

Anti-HIV CRISPR (Prof. Ben Berkhout)

5th Day

CRISPR screening

09:00 — 10:15

CRISPR-based genetic screens (Dr. Kosuke Yusa)

10:15 — 11:30

CRISPR screens in vivo (Dr. Sidi Chen)

11:30 — 12:45

Development and application of CRISPR/CAS9 technologies for large scale pooled screening (Dr. Roderick Beijersbergen)

12:45 — 14:15

Lunch

14:15 — 16:15

Free afternoon

16:15 — 18:15

Hiking on Lamma Island (if weather permits)

6th Day

CRISPR applications

09:00 — 10:15

Antiviral CRISPR (Prof. Ben Berkhout)

10:15 — 11:30

Discovery of therapeutic targets and drug resistance mechanisms by functional genetic screening (Dr. Andrew Aguirre)

11:30 — 12:45

Defining the genome-wide activity of CRIPR-Cas nucleases for therapeutics (Dr. Shengdar Tsai)

12:45 — 14:25

Lunch

14:25 — 16:55

Practical Session 4: 1) PCR verification and data analysis. 2) Single cell isolation. 3) Cell sorting demonstration.

16:55 — 18:25

Concluding remarks (Prof. Dong-Yan Jin and Prof. Ben Berkhout)

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Stay updated with the latest scientific developments in Hong Kong through Croucher News.