Programme
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10 August – 15 August 2018
Past
1st Day
Introduction to CRISPR
09:00 – 09:15
Opening remarks (Prof. Dong-Yan Jin and Prof. Ben Berkhout)
09:15 – 10:30
Introduction to CRISPR (Dr. Bo Gao)
10:30 – 11:45
Cutting-edge CRISPR technologies (Dr. Alan Wong)
11:45 – 13:00
Bioinformatics of gRNA design (Prof. Qi Liu)
13:00 – 14:30
Lunch
14:30 – 17:00
Practical session 1: 1) Practical briefing. 2) gRNA design. 3) Transfection of CRISPR plasmids into HEK293 cells (Dr. Sam Yuen)
2nd Day
CRISPR in model systems
09:00 – 10:30
Genome editing in preimplantation embryos and in EPSCs (Prof. Pengtao Liu)
10:30 – 12:20
Organoids as a model system and their tools for genetic manipulation (Dr. Bon-Kyoung Koo)
12:20 – 14:30
Lunch
14:30 – 17:00
Practical Session 2: 1) Cloning of CRISPR construct. 2) Introduction to CRISPR library construction and other CRISPR resources (Dr. Sam Yuen)
3rd Day
Mechanism and delivery of CRISPR
09:00 – 10:30
Crystallography and HF-AFM of CRISPR-Cas9 (Prof. Osamu Nureki)
10:30 – 12:20
Workshop (Prof. Ben Berkhout and all invited speakers)
12:20 – 14:30
Lunch
14:30 – 17:00
Practical Session 3: 1) Harvest of genomic DNA. 2) PCR verification of CRISPR editing (Dr. Sam Yuen)
4th Day
Symposium on precision genome editing
08:30 – 08:45
Opening remarks (HKU representatives)
08:45 – 09:30
Bioinformatics of CRISPR editing (Dr. Qi Liu)
09:30 – 10:25
Molecular mechanism of CRISPR-Cas and structure-guided development of genome-editing tools towards medical applications (Prof. Osamu Nureki)
10:25 – 11:10
CRISPR editing in mice (Prof. Pengtao Liu)
11:10 – 11:55
CRISPR/Cas-assisted genetics in intestinal organoids (Dr. Bon-Kyoung Koo)
11:55 – 12:40
CRISPR-based genetic screens (Dr. Kosuke Yusa)
12:40 – 14:00
Lunch
14:00 – 14:45
Gene editing of the functional cancer genome (Dr. Sidi Chen)
14:45 – 15:40
The use of large scale functional genomic screens for the identification of novel drug targets and effective combination therapies (Dr. Roderick Beijersbergen)
15:40 – 16:25
CRISPR-Cas9 screening to identify cancer dependencies (Dr. Andrew Aguirre)
16:25 – 17:10
CRISPR-Cas genome editing technologies for therapeutics (Dr. Shengdar Tsai)
17:10 – 17:55
Anti-HIV CRISPR (Prof. Ben Berkhout)
5th Day
CRISPR screening
09:00 – 10:15
CRISPR-based genetic screens (Dr. Kosuke Yusa)
10:15 – 11:30
CRISPR screens in vivo (Dr. Sidi Chen)
11:30 – 12:45
Development and application of CRISPR/CAS9 technologies for large scale pooled screening (Dr. Roderick Beijersbergen)
12:45 – 14:15
Lunch
14:15 – 16:15
Free afternoon
16:15 – 18:15
Hiking on Lamma Island (if weather permits)
6th Day
CRISPR applications
09:00 – 10:15
Antiviral CRISPR (Prof. Ben Berkhout)
10:15 – 11:30
Discovery of therapeutic targets and drug resistance mechanisms by functional genetic screening (Dr. Andrew Aguirre)
11:30 – 12:45
Defining the genome-wide activity of CRIPR-Cas nucleases for therapeutics (Dr. Shengdar Tsai)
12:45 – 14:25
Lunch
14:25 – 16:55
Practical Session 4: 1) PCR verification and data analysis. 2) Single cell isolation. 3) Cell sorting demonstration.
16:55 – 18:25
Concluding remarks (Prof. Dong-Yan Jin and Prof. Ben Berkhout)
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