Programme
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8 August — 13 August 2025
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University of Hong Kong
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HK$5,000 (Includes accommodation)
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Apply by 11 July 2025
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Fully booked
1st Day
Introduction to CRISPR
09:30 — 09:40
Opening remarks by Professor Dongyan Jin
09:40 — 10:55
Introduction to CRISPR by Professor Alan Wong
10:55 — 11:10
Tea break
11:10 — 12:30
Recombinase-controlled multiphase condensates for CRISPR diagnostics
12:30 — 14:30
Lunch
14:30 — 16:30
Practical session 1
1. Briefing on CRISPR technology
2. Design of gRNAs
3. CRISPR editing analysis
2nd Day
Theme and variations
09:30 — 10:45
Technical design in type I cascade-mediated genetic exploitations by Professor Aixin Yan
10:45 — 11:00
Tea break
11:00 — 12:20
Directed evolution of adenine base editors: Tackling its applications for human gene therapy
12:20 — 14:30
Lunch
14:30 — 16:30
Practical session 2
1. Introduction to CRISPR screening
2. CRISPR library preparation
3rd Day
Emerging technologies of precision editing
09:30 — 10:45
Development and application of high-precision gene editing technologies
10:45 — 11:00
Tea break
11:00 — 12:20
Development and characterization of precision base editor tools
12:20 — 14:00
Lunch
14:00 — 15:00
Free afternoon
15:00 — 17:00
West Kowloon Cultural District visit
4th Day
CRISPR in different models
09:30 — 10:45
From organoid genetics to mosaic genetics
10:45 — 11:00
Tea break
11:00 — 12:20
Population-scale cellular GUIDE-seq-2 and biochemical CHANGE-seq-R profiles reveal human genetic variation frequently affects Cas9 off target activity
12:20 — 14:30
Lunch
14:30 — 18:30
Practical session 3
1. Harvest and purify the library plasmid pool
2. PCR verification of CRISPR editing
5th Day
New frontiers
09:30 — 10:50
New frontiers in CRISPR-Cas, RNA-guided proteases and ancestral Cas9s in transposons
10:50 — 11:00
Tea break
11:00 — 12:20
Epigenome editing to regulate gene expression
12:20 — 14:25
Lunch
14:25 — 16:55
Practical session 4
1. Introduction to CRISPR screening strategies
2. Cell sorting demonstration
16:55 — 17:10
Concluding remarks by Professor Dongyan Jin
6th Day
One-day symposium on precision genome engineering
09:20 — 09:30
Opening remarks
09:30 — 10:20
CRISPR diagnostics for infectious diseases: from the clinic to the basics
10:20 — 11:10
Organoid zoo: genome engineering in organoids
11:10 — 11:20
Tea break
11:20 — 12:10
Directed evolution of adenine base editors: Tackling its applications for human gene therapy
12:10 — 13:00
Development and application of high-precision gene editing technologies
13:00 — 14:00
Lunch time technical seminar
14:00 — 14:50
Leveraging molecular mechanisms in CRISPR-Cas systems for genome editing applications
14:50 — 15:40
Understanding human genetic variation with precision genome editing
15:40 — 15:50
Tea break
15:50 — 16:40
CRISPR genome editing technology and therapeutics for sickle cell disease
16:40 — 17:30
Advancing personalized genome edited human hematopoietic stem cell therapies: From sickle cell disease to bone marrow failure disorders
Other courses
Information theory
3
–
8
August
2025
Chinese University of Hong Kong
Fully booked
Neutron scattering
10
–
16
August
2025
City University of Hong Kong
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