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Projects and events Summer Courses Precision genome engineering

Programme

  • 8 August — 13 August 2025

  • University of Hong Kong

  • HK$5,000 (Includes accommodation)

  • Apply by 1 May 2025

1st Day
Introduction to CRISPR
09:30 — 09:40
Opening remarks
09:40 — 10:55
Introduction to CRISPR
10:55 — 11:10
Tea break
11:10 — 12:30
Genome-wide CRISPR screens in human immune cells to dissect host-pathogen interactions
12:30 — 14:30
Lunch
14:30 — 16:30
Practical session 1

1. Briefing on CRISPR technology

2. Design of gRNAs

3. CRISPR editing analysis

2nd Day
CRISPR in action
09:30 — 10:45
Tips and tricks to perform genome engineering in adult stem cells and organoids
10:45 — 11:00
Tea break
11:00 — 12:20
CRISPR prime editing
12:20 — 14:30
Lunch
14:30 — 16:30
Practical session 2

1. Introduction to CRISPR screening 

2. CRISPR library preparation

3rd Day
Type I CRISPR-Cas system
09:30 — 10:45
Mechanism of Type-I CRISPR-Cas system and its preliminary application
10:45 — 11:00
Tea break
11:00 — 12:20
Technical design in type I cascade-mediated genetic exploitations
12:20 — 14:30
Lunch
14:30 — 16:30
Practical session 3

1. Harvest and purify the library plasmid pool 

2. PCR verification of CRISPR editing

4th Day
CRISPR in different models
09:30 — 10:45
Knocking down RNAs using CRISPR-Cas13
10:45 — 11:00
Tea break
11:00 — 12:20
Genome editing in the zygote and expanded potential stem cells
12:20 — 14:00
Lunch
14:00 — 18:00
Exploring West Kowloon Cultural District
5th Day
CRISPR applications: Safety and ethics
09:30 — 10:50
CRISPR-Cas, CAR T cells and other major gene therapy activities
10:50 — 11:00
Tea break
11:00 — 12:20
Understanding the safety or genome-wide activity of CRISPR genome editors
12:20 — 14:25
Lunch
14:25 — 16:55
Practical session 4

1. Introduction to CRISPR screening strategies

2. Cell sorting demonstration

16:55 — 17:10
Concluding remarks
6th Day
One-day symposium on precision genome engineering
08:30 — 08:40
Opening remarks
08:40 — 09:30
Native class 1 type I CRISPR-Cas systems in prokaryotes: from in situ genome editing to autoimmunity
09:30 — 10:20
How type-II CRISPR-Cas9 system establishes immunity in bacteria
10:20 — 10:30
Tea break
10:30 — 11:20
Genome editing in expanded potential stem cells
11:20 — 12:10
CRISPR-based genome engineering in organoids
12:10 — 13:00
Enhancing CRISPR-Cas with artificial intelligence and protein engineering
13:00 — 14:00
Lunch time technical seminar
14:00 — 14:50
CRISPR-based technologies for living medicines and microbiome engineering
14:50 — 15:40
RNA-targeting CRISPR-Cas systems
15:40 — 15:50
Tea break
15:50 — 16:40
CRISPR delivery: approaches and challenges
16:40 — 17:30
CRISPR genome editing technology and therapeutics for sickle cell disease
17:30 — 18:30
CRISPR attack on HIV DNA for a cure

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