CRISPR-mediated knockout of target genes has become the standard for loss-of-function studies in mammalian cells and animals. CRISPR-based forward genetic screens and other applications of CRISPR including those in live cell imaging, disease modelling and treatment have brought revolutionary thoughts and enabling tools to various fields across biology and medicine. The CRISPR revolution is not only in the lab but will also be in the clinic. The approval for the first CRISPR gene therapy is pending. However, there is a big gap between the rapid development of CRISPR and its potential applications in the local and the wider region, where it is still much underused. The goal of our course is to fill this gap by educating our young scientists with cutting-edge technologies, providing a forum and platform for discussion and knowledge exchange, promoting wider use of CRISPR, and strengthening our link to the world. Our course will not only introduce state-of-the-art developments but also provide hands-on training.