Canada Research Chair in Electron Cryomicroscopy (Tier I) (2014-2021) Professor Departments of Biochemistry and Medical Biophysics University of Toronto Expertise: Single-particle cryo-EM and cryo-EM method development John Rubinstein obtained his B.Sc from the University of Guelph in 1998. He received his PhD from Cambridge University (2002) where he worked in Medical Research Council laboratories under the supervision of Sir John E. Walker and Dr. Richard Henderson. Dr. Rubinstein was a Postdoctoral Research Associate at the MRC Laboratory of Molecular Biology before returning to Canada for a National Cancer Institute of Canada (NCIC) postdoctoral fellowship at the Banting and Best Department of Medical Research. He joined the Research Institute of The Hospital for Sick Children in 2006. He is a Professor in the Departments of Medical Biophysics and Biochemistry at the University of Toronto, and a Tier 1 Canada Research Chair in Electron Cryomicroscopy. His accomplishments have been recognized by awards including a CIHR New Investigator Award, the GE Healthcare New Investigator Award from the Canadian Society for Molecular Biosciences, and the Burton Medal from the Microscopy Society of America. The Rubinstein laboratory studies the structure and function of macromolecular assemblies using electron cryomicroscopy (cryo-EM), image analysis, molecular biology and molecular genetics. They also develop the tools of cryo-EM so that they can answer questions that are not amenable to the techniques that currently exist. Method development usually occurs on several levels: (1) Development of new algorithms and computational approaches for image analysis (e.g. Rubinstein and Brubaker, 2015); and (2) Nanofabrication to improve specimen preparation (e.g. Marr et al., 2014). For computational method development they collaborate closely with colleagues in the Department of Computer Sciences at the University of Toronto. Recent biological projects have included the structural study of the mitochondrial ATP synthase (Zhou et al., 2015), the Vacuolar-type ATPase (Zhao et al., 2015), and the V/A-ATPase (Lau and Rubinstein, 2012). These rotary ATPases have important roles in cancer, osteoporosis, the immune system, and ischemia-reperfusion injury. They have numerous collaborative projects studying other aspects of the molecular biology of the cell from a structural perspective. Their work on the rotary ATPases and other ongoing projects has illustrated how cryo-EM can be used not only to elucidate the high-resolution structures of macromolecular assemblies, but also to understand the dynamics of biomolecular systems List of Key Publications :   1. Guo H, Bueler SA and Rubinstein JL* (2017) Atomic model for the dimeric FO region of mitochondrial ATP synthase. Science, eaao4815. 2. Ereño-Orbea J, Taylor Sicard HC, Mazhab-Jafari MT, Benlekbir S, Guarné A, Rubinstein JL and Julien JP (2017) Molecular basis of human CD22 function and therapeutic targeting. Nature communications, 8. 3. Zhou A, Rohou A, Schep DG, Bason JV, Montgomery MG, Walker JE, Grigorieff N and Rubinstein JL* (2015) Structure and conformational states of the bovine mitochondrial ATP synthase by cryo-EM. eLife 4:e10180 [BioRxiv:11 Aug 2015] 4. Zhao J, Benlekbir S and Rubinstein JL* (2015). Cryo-EM observation of rotational states in a eukaryotic V-ATPase. Nature 521, 241-5. 5. Smith MTJ and Rubinstein JL* (2014). Beyond blob-ology. Science 345, 617-9.