The project focused on developing novel diagnostics and therapeutics for metabolic tissue regeneration. Adult stem cells, capable of differentiating into multiple cell types, hold potential as regenerative therapies. However, these cells are scarce and activate rapidly when dissociated from their niche. This study combined the Laboratory of Cheung and Zeng, which specialised in satellite cells from skeletal muscle and pancreatic progenitor cells respectively. The joint laboratory investigated the role of adult stem cells in metabolic tissue regeneration.

The team established an organoid-based culture system derived from adult stem and niche cells in a multi-omics approach to understand tissue damage and repair. They explored molecular mechanisms of quiescence and activation in satellite and pancreatic progenitor cells, and identified genes encoding transcription factors that support stem cell functions, which were validated in the organoid system and in vivo. Phenotypic screening was used to identify small molecules that enhance these transcription factors, which discovered therapeutic drugs for metabolic tissue regeneration.