Frontier in Cellular Biology

This summer course aims to provide the most up-to-date training for postgraduate students and early scientist in cell and integrative biology technology and platform by using the state-of-the-art platforms. 

Monday 1 July

09:30

Lab 1: in vitro budding reconstitution techniques and Patch-clamp techniques

12:20

Lunch and discussion

14:30

Lab 2: Advanced cell biology techniques and Imaris Software analysis

17:30

Dinner and discussion

19:00

Evening discussion and data analysis for presentation

Tuesday 2 July

09:30

Technical Lecture A: Professor David G Drubin

09:30

Technical Lecture B: Professor Ralph Bock

10:00

Tea break

10:40

Technical Lecture C: Professor Randy Schekman

11:10

Technical Lecture D: Professor Jen Sheen

11:45

Lunch and discussion

14:00

Lab 3: Ultrastructural study of transport vesicles and organelles in plant and animal cells

17:00

Dinner and discussion

18:30

Evening informal chatting with speakers on scientific research and career development (I)

Wednesday 3 July

09:30

Opening remarks and group photo

09:50

Lecture 1: Professor David G Drubin

10:40

Lecture 2: Professor Ralph Bock

11:30

Lecture 3: Professor Randy Schekman

12:20

Lunch and discussion

13:40

Lecture 4: Professor Jen Sheen

14:30

Lecture 5: Professor Min Wu

15:20

Tea break

16:00

Lecture 6: Professor Justin Taraska

16:50

Lecture 7

17:30

Dinner and discussion

19:00

Evening informal chatting with speakers on scientific research and career development (II)

Thursday 4 July

09:30

Technical Lecture E: Professor Min Wu

10:00

Technical Lecture F: Professor Justin Taraska

10:30

Tea break

11:10

Technical Lecture

11:40

Discussion

12:15

Lunch and discussion

14:30

Lab 4: Plant transient expression systems and demonstration on Cryo-TE/ET Analysis

17:30

Dinner and discussion

19:00

Evening discussion and data analysis for presentation

Friday 5 July

09:30

Lab 5: Hands on training

12:15

Lunch and discussion

14:30

Students' presentation and awards presentations

17:30

Dinner and social activities

Speakers

  • Professor Randy SCHEKMAN Professor, Department of Molecular & Cell Biology, University of California, Berkeley Editor-in-Chief, eLife Principle Investigator, Howard Hughes Medical Institute Member of National Academy of Sciences of USA Professor Department of Molecular & Cell Biology, University of California at Berkeley, USA Expertise: Protein Sorting and Disease Connections in the Secretory Pathway of Yeast and Mammalian Cells Research interests: 1) Organelle assembly, 2) Intracellular protein transport, 3) Assembly of cellular organelles, 4) Alzheimer's Disease Publications:  COPII-coated membranes function as transport carriers of intracellular procollagen I. Gorur A, Yuan L, Kenny SJ, Baba S, Xu K, Schekman R. J Cell Biol. 2017 Apr 20. pii: jcb.201702135. doi: 10.1083/jcb.201702135.  Remodeling of ER-exit sites initiates a membrane supply pathway for autophagosome biogenesis. Ge L, Zhang M, Kenny SJ, Liu D, Maeda M, Saito K, Mathur A, Xu K, Schekman R. EMBO Rep. 2017 Jul 28. pii: e201744559. doi: 10.15252/embr.201744559.  Neurodegeneration-associated mutant TREM2 proteins abortively cycle between the ER and ER-Golgi intermediate compartment. Sirkis DW, Aparicio RE, Schekman R. Mol Biol Cell. 2017 Aug 2. pii: mbc.E17-06-0423. doi: 10.1091/mbc.E17-06-0423.  TFG facilitates outer coat disassembly on COPII transport carriers to promote tethering and fusion with ER-Golgi intermediate compartments. Hanna MG 4th, Block S, Frankel EB, Hou F, Johnson A, Yuan L, Knight G, Moresco JJ, Yates JR 3rd, Ashton R, Schekman R, Tong Y, Audhya A. Proc Natl Acad Sci U S A. 2017 Sep 12;114(37):E7707-E7716. doi: 10.1073/pnas.1709120114. Epub 2017 Aug 29.  Broad role for YBX1 in defining the small noncoding RNA composition of exosomes. Shurtleff MJ, Yao J, Qin Y, Nottingham RM, Temoche-Diaz MM, Schekman R, Lambowitz AM. Proc Natl Acad Sci U S A. 2017 Oct 24;114(43):E8987-E8995. doi: 10.1073/pnas.1712108114. Epub 2017 Oct 10.
  • Professor David DRUBIN Professor of Cell & Developmental Biology, University of California, Berkeley (Associate Director for this CSC ) Fellow of American Academy of Arts and Sciences Editor-in-Chief, Molecular Biology of the Cell (MBoC) Professor of Cell & Developmental Biology Department of Molecular & Cell Biology, University of California at Berkeley, USA Expertise: Actin Dynamics and Endocytic Trafficking in Yeast and Mammalian Cells Publications  Kinesins relocalize the chromosomal passenger complex to the midzone for spindle disassembly. Ibarlucea-Benitez I, Ferro LS, Drubin DG, Barnes G. J Cell Biol. 2018 May 7;217(5):1687-1700. doi: 10.1083/jcb.201708114. Epub 2018 Mar 21.  The Scientific Society Publisher Alliance delivers an important message. Drubin DG. Mol Biol Cell. 2017 Dec 1;28(25):3563. doi: 10.1091/mbc.E17-11-0628  Switch-like Arp2/3 activation upon WASP and WIP recruitment to an apparent threshold level by multivalent linker proteins in vivo. Sun Y, Leong NT, Jiang T, Tangara A, Darzacq X, Drubin DG. Elife. 2017 Aug 16;6. pii: e29140. doi: 10.7554/eLife.29140.  Selection and stabilization of endocytic sites by Ede1, a yeast functional homologue of human Eps15. Lu R, Drubin DG. Mol Biol Cell. 2017 Mar 1;28(5):567-575. doi: 10.1091/mbc.E16-06-0391. Epub 2017 Jan 5.  The mismeasure of scientific research articles and why MBoC quickly embraced preprints. Drubin DG. Mol Biol Cell. 2016 Nov 1;27(21):3181-3182
  • Professor Elliot MEYEROWITZ (Pending) Principle Investigator, Howard Hughes Medical Institute, California Institute of Technology Principle Investigator, Howard Hughes Medical Institute Member of National Academy of Sciences of USA George W. Beadle Professor of Biology Division of Biology California Institute of Technology, USA Expertise: Flower Development and Arabidopsis as a Model for Developmental Study Research interests: 1) To understand how stem cell populations are maintained 2) How these cell populations form organized and differentiated structures. Publications:  SUPERMAN regulates floral whorl boundaries through control of auxin biosynthesis. Xu Y, Prunet N, Gan ES, Wang Y, Stewart D, Wellmer F, Huang J, Yamaguchi N, Tatsumi Y, Kojima M, Kiba T, Sakakibara H, Jack TP, Meyerowitz EM, Ito T. EMBO J. 2018 May 15. pii: e97499. doi: 10.15252/embj.201797499.  Transcriptome dynamics at Arabidopsis graft junctions reveal an intertissue recognition mechanism that activates vascular regeneration. Melnyk CW, Gabel A, Hardcastle TJ, Robinson S, Miyashima S, Grosse I, Meyerowitz EM. Proc Natl Acad Sci U S A. 2018 Mar 6;115(10):E2447-E2456. doi: 10.1073/pnas.1718263115. Epub 2018 Feb 13.  Cell Cycle Control by Nuclear Sequestration of CDC20 and CDH1 mRNA in Plant Stem Cells. Yang W, Wightman R, Meyerowitz EM. Mol Cell. 2017 Dec 21;68(6):1108-1119.e3. doi: 10.1016/j.molcel.2017.11.008. Epub 2017 Dec 7.  Field Guide to Plant Model Systems. Chang C, Bowman JL, Meyerowitz EM. Cell. 2016 Oct 6;167(2):325-339. doi: 10.1016/j.cell.2016.08.031.  Genetics and plant development. Prunet N, Meyerowitz EM. C R Biol. 2016 Jul-Aug;339(7-8):240-6. doi: 10.1016/j.crvi.2016.05.003. Epub 2016 May 26
  • Professor Ralph BOCK Managing Director, Max Planck Institute of Molecular Plant Physiology (MPI-MP) Director of Department 3: Organelle Biology, Biotechnology and Molecular Ecophysiology Max Planck Institute of Molecular Plant Physiology Expertise: Chloroplast Signaling and metabolic engineering Research interests: 1) Chloroplast Signaling 2) Plastid transformation Publications:  Chloroplast Translation: Structural and Functional Organization, Operational Control, and Regulation. Zoschke R, Bock R. Plant Cell. 2018 Apr;30(4):745-770. doi: 10.1105/tpc.18.00016. Epub 2018 Apr 2  Control of Retrograde Signaling by Rapid Turnover of GENOMES UNCOUPLED1. Wu GZ, Chalvin C, Hoelscher M, Meyer EH, Wu XN, Bock R. Plant Physiol. 2018 Mar;176(3):2472-2495. doi: 10.1104/pp.18.00009. Epub 2018 Jan 24.  Temporal Proteomics of Inducible RNAi Lines of Clp Protease Subunits Identifies Putative Protease Substrates. Moreno JC, Martínez-Jaime S, Schwartzmann J, Karcher D, Tillich M, Graf A, Bock R. Plant Physiol. 2018 Feb;176(2):1485-1508. doi: 10.1104/pp.17.01635. Epub 2017 Dec 11.  Loopholes for smuggling DNA into pollen. Ruf S, Bock R. Nat Plants. 2017 Dec;3(12):918-919. doi: 10.1038/s41477-017-0072-y.  Shine-Dalgarno Sequences Play an Essential Role in the Translation of Plastid mRNAs in Tobacco. Scharff LB, Ehrnthaler M, Janowski M, Childs LH, Hasse C, Gremmels J, Ruf S, Zoschke R, Bock R. Plant Cell. 2017 Dec;29(12):3085-3101. doi: 10.1105/tpc.17.00524. Epub 2017 Nov 13.
  • Professor Julian SCHROEDER (Pending) Distinguished Professor, University of California, San Diego Distinguished Professor, Section of Cell and Developmental Biology, University of California at San Diego, USA Expertise: signal transduction mechanisms and pathways that mediate resistance to environmental stresses in plants Research interests: 1) Signal transduction mechanisms and pathways that mediate resistance to environmental stresses in plants 2) Identifying genes that mediate salt (sodium) stress resistance and heavy metal uptake and detoxification in plants Publications:  Abscisic acid-induced degradation of Arabidopsis guanine nucleotide exchange factor requires calcium-dependent protein kinases. Li Z, Takahashi Y, Scavo A, Brandt B, Nguyen D, Rieu P, Schroeder JI. Proc Natl Acad Sci U S A. 2018 May 8;115(19):E4522-E4531. doi: 10.1073/pnas.1719659115. Epub 2018 Apr 23.  SnapShot: Abscisic Acid Signaling. Hauser F, Li Z, Waadt R, Schroeder JI. Cell. 2017 Dec 14;171(7):1708-1708.e0. doi: 10.1016/j.cell.2017.11.045.  Screening for Natural Variation in Water Use Efficiency Traits in a Diversity Set of Brassica napus L. Identifies Candidate Variants in Photosynthetic Assimilation. Pater D, Mullen JL, McKay JK, Schroeder JI. Plant Cell Physiol. 2017 Oct 1;58(10):1700-1709.  Seeing is believing. Azoulay-Shemer T, Hsu PK, Schroeder JI. Nat Plants. 2017 Oct;3(10):765-766. doi: 10.1038/s41477-017-0025-5.  Two-electrode Voltage-clamp Recordings in Xenopus laevis Oocytes: Reconstitution of Abscisic Acid Activation of SLAC1 Anion Channel via PYL9 ABA Receptor. Wang C, Zhang J, Schroeder JI. Bio Protoc. 2017 Jan 20;7(2). pii: 2114. doi: 10.21769/BioProtoc.2114.
  • Professor Sabeeha MERCHANT (Pending) Distinguished Professor, University of California Los Angeles Distinguished Professor, Biochemistry, Molecular Biology University of California at Los Angeles, USA Expertise: uses elemental and protein mass spectrometry in combination with live cell imaging of metal sensors and classical genetics to discover and dissect the biochemistry of the metal storing compartment. Research interests: 1) Genomics of algae, 2) Mineral metabolism in algae and plants, 3) Impact of mineral deficiency on photosynthesis, 4) Metabolism of trace elements, 5) Regulation of accumulation of biofuel precursors in algae Publications:  Using YFP as a Reporter of Gene Expression in the Green Alga Chlamydomonas reinhardtii. Blaby-Haas CE, Page MD, Merchant SS. Methods Mol Biol. 2018;1755:135-148.  The Plant Cell Welcomes Assistant Features Editors. Eckardt NA, Williams M, Merchant SS. Plant Cell. 2018 Jan;30(1):1-2. doi: 10.1105/tpc.18.00048. Epub 2018 Jan 19.  High-throughput sequencing of the chloroplast and mitochondrion of Chlamydomonas reinhardtii to generate improved de novo assemblies, analyze expression patterns and transcript speciation, and evaluate diversity among laboratory strains and wild isolates. Gallaher SD, Fitz-Gibbon ST, Strenkert D, Purvine SO, Pellegrini M, Merchant SS. Plant J. 2018 Feb;93(3):545-565. doi: 10.1111/tpj.13788. Epub 2018 Jan 7.  Regulating cellular trace metal economy in algae. Blaby-Haas CE, Merchant SS. Curr Opin Plant Biol. 2017 Oct;39:88-96. doi: 10.1016/j.pbi.2017.06.005. Epub 2017 Jun 30.
  • Professor Dominique C. BERGMANN (Pending) Associate Professor, Stanford University Associate Professor, Stanford University, USA Expertise: Cell and Developmental Biology Research interests: 1) Study of cell fate 2) Stem cell self-renewal 3) Cell polarity in plants. Publications:  Dissection of MAPK signaling specificity through protein engineering in a developmental context. Wengier DL, Lampard GR, Bergmann DC. BMC Plant Biol. 2018 Apr 10;18(1):60. doi: 10.1186/s12870-018-1274-9.  Direct Control of SPEECHLESS by PIF4 in the High-Temperature Response of Stomatal Development. Lau OS, Song Z, Zhou Z, Davies KA, Chang J, Yang X, Wang S, Lucyshyn D, Tay IHZ, Wigge PA, Bergmann DC. Curr Biol. 2018 Apr 23;28(8):1273-1280.e3. doi: 10.1016/j.cub.2018.02.054. Epub 2018 Apr 5.  Lineage- and stage-specific expressed CYCD7;1 coordinates the single symmetric division that creates stomatal guard cells. Weimer AK, Matos JL, Sharma N, Patell F, Murray JAH, Dewitte W, Bergmann DC. Development. 2018 Mar 21;145(6). pii: dev160671. doi: 10.1242/dev.160671.  Disruption of stomatal lineage signaling or transcriptional regulators has differential effects on mesophyll development, but maintains coordination of gas exchange. Dow GJ, Berry JA, Bergmann DC. New Phytol. 2017 Oct;216(1):69-75. doi: 10.1111/nph.14746. Epub 2017 Aug 21  Mobile MUTE specifies subsidiary cells to build physiologically improved grass stomata. Raissig MT, Matos JL, Anleu Gil MX, Kornfeld A, Bettadapur A, Abrash E, Allison HR, Badgley G, Vogel JP, Berry JA, Bergmann DC. Science. 2017 Mar 17;355(6330):1215-1218. doi: 10.1126/science.aal3254.