Friday 10 August: Introduction to CRISPR
09:00
Opening remarks (Prof. Dong-Yan Jin and Prof. Ben Berkhout)
09:15
Introduction to CRISPR (Dr. Bo Gao)
10:30
Cutting-edge CRISPR technologies (Dr. Alan Wong)
11:45
Bioinformatics of gRNA design (Prof. Qi Liu)
13:00
Lunch
14:30
Practical session 1: 1) Practical briefing. 2) gRNA design. 3) Transfection of CRISPR plasmids into HEK293 cells (Dr. Sam Yuen)
Saturday 11 August: CRISPR in model systems
09:00
Genome editing in preimplantation embryos and in EPSCs (Prof. Pengtao Liu)
10:30
Organoids as a model system and their tools for genetic manipulation (Dr. Bon-Kyoung Koo)
12:20
Lunch
14:30
Practical Session 2: 1) Cloning of CRISPR construct. 2) Introduction to CRISPR library construction and other CRISPR resources (Dr. Sam Yuen)
Sunday 12 August: Mechanism and delivery of CRISPR
09:00
Crystallography and HF-AFM of CRISPR-Cas9 (Prof. Osamu Nureki)
10:30
Workshop (Prof. Ben Berkhout and all invited speakers)
12:20
Lunch
14:30
Practical Session 3: 1) Harvest of genomic DNA. 2) PCR verification of CRISPR editing (Dr. Sam Yuen)
Monday 13 August: Symposium on precision genome editing
08:30
Opening remarks (HKU representatives)
08:45
Bioinformatics of CRISPR editing (Dr. Qi Liu)
09:30
Molecular mechanism of CRISPR-Cas and structure-guided development of genome-editing tools towards medical applications (Prof. Osamu Nureki)
10:25
CRISPR editing in mice (Prof. Pengtao Liu)
11:10
CRISPR/Cas-assisted genetics in intestinal organoids (Dr. Bon-Kyoung Koo)
11:55
CRISPR-based genetic screens (Dr. Kosuke Yusa)
12:40
Lunch
14:00
Gene editing of the functional cancer genome (Dr. Sidi Chen)
14:45
The use of large scale functional genomic screens for the identification of novel drug targets and effective combination therapies (Dr. Roderick Beijersbergen)
15:40
CRISPR-Cas9 screening to identify cancer dependencies (Dr. Andrew Aguirre)
16:25
CRISPR-Cas genome editing technologies for therapeutics (Dr. Shengdar Tsai)
17:10
Anti-HIV CRISPR (Prof. Ben Berkhout)
Tuesday 14 August: CRISPR screening
09:00
CRISPR-based genetic screens (Dr. Kosuke Yusa)
10:15
CRISPR screens in vivo (Dr. Sidi Chen)
11:30
Development and application of CRISPR/CAS9 technologies for large scale pooled screening (Dr. Roderick Beijersbergen)
12:45
Lunch
14:15
Free afternoon
16:15
Hiking on Lamma Island (if weather permits)
Wednesday 15 August: CRISPR applications
09:00
Antiviral CRISPR (Prof. Ben Berkhout)
10:15
Discovery of therapeutic targets and drug resistance mechanisms by functional genetic screening (Dr. Andrew Aguirre)
11:30
Defining the genome-wide activity of CRIPR-Cas nucleases for therapeutics (Dr. Shengdar Tsai)
12:45
Lunch
14:25
Practical Session 4: 1) PCR verification and data analysis. 2) Single cell isolation. 3) Cell sorting demonstration.
16:55